EXPLORING THE ANTIOXIDANT POTENTIAL OF ARGYREIA IMBRICATA: A BIOCHEMICAL ANALYSIS

Abstract

Reactive oxygen species (ROS) play a substantial role in the pathophysiology of a variety of diseases. An excess of the ROS produced by metabolic activities, such as superoxide radical anion(SOR), hydrogen peroxide(H2O2), hydroxyl radical(OH), and nitric oxide(NO), causes inflammatory responses. Antioxidants protect cells from cellular damage caused by reactive oxygen species. The most popular "antioxidants" are vitamins A(retinol), C(L-ascorbic acid), β-carotene, minerals like selenium, and naturally occurring polyphenols. Antioxidants are present in the diet, and some people additionally take antioxidant supplements. The ingestion of natural antioxidants will reduce the risk of cardiovascular disease, diabetes and other diseases of cancer associated with ageing. Nature has abundant source of antioxidant availability present in this study we have investigated Argyreia imbricata leaves aqueous extract(AIAE) for antioxidant activity and lipid peroxidation assay. The aqueous extract showed the maximum antioxidant activity, as measured by total antioxidant activity(TAA) of 1.32µg/ml and ferric reducing antioxidant power of 16.36µg/ml. Furthermore, aqueous extract has shown the greatest DPPH radical scavenging activity with an IC50 value of 33.16µg/ml, ABTS radical cation decolourisation assay with a value of 20.85µg/ml, nitric oxide scavenging assay with a value of 26.89µg/ml and maximum inhibition of lipid peroxidation. In conclusion, the AIAE leaves was analysed for antioxidant activities, in which the extract exhibited significant activity. In conclusion, this biochemical study highlights the antioxidant and lipid peroxidation properties of A.imbricata leaves extract, suggesting its potential as a natural therapeutic agent in combating oxidative stress-related disorders.